Embryos were then treated in 10 µg/ml proteinase k in pbs-tween for 3 min, incubated in glycine in pbs-tween, washed in pbs-tween and finally re-fixed for 20 min with 4% pfa in pbs, 0.2%.
Diabetes Smarts Video Presentation :
Embryos were then treated in 10 µg/ml proteinase k in pbs-tween for 3 min, incubated in glycine in pbs-tween, washed in pbs-tween and finally re-fixed for 20 min with 4% pfa in pbs, 0.2%.
